Journal: Frontiers in Cellular Neuroscience

Article Title: Chronic Tear Deficiency Sensitizes Transient Receptor Potential Vanilloid 1-Mediated Responses in Corneal Sensory Nerves

doi: 10.3389/fncel.2020.598678

Figure Lengend Snippet: Photomicrographs of FM 1-43FX-positive corneal neurons and glial fibrillary acidic protein (GFAP)-immunoreactive cells in the anteromedial part of the trigeminal ganglion. (A,B) The trigeminal ganglion sections of the sham-operated (A) and gland excision guinea pigs (B) . The corneal neurons were labeled with FM 1-43FX (green in the upper images), and the trigeminal ganglia were subsequently immunostained with anti-GFAP antibody (red in the middle images). The merged FM1-43FX/GFAP images are represented in the lower panels. The white arrows represent the FM 1-43FX-positive corneal neurons. Scale bars = 50 μm. (C) Percentage of FM 1-43FX-positive neurons completely (>80%) or partially (20–80%) enclosed by the GFAP-immunoreactive structure in the total FM 1-43FX-positive neurons. * P < 0.05 vs. the sham-operated group (Student’s t -test).

Article Snippet: Subsequently, the sections were incubated with mouse anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1,000, GeneTex, #GTX40996, Irvine, CA, USA) diluted with 0.01 M PBS containing 4% normal goat serum and 0.3% Triton X 100 for 24 h at 4°C.

Techniques: Labeling